lens 32  (Nikon)

Journal: STAR Protocols

Article Title: Protocol for controlling visual experience during zebrafish development and modulation of motor behavior

doi: 10.1016/j.xpro.2023.102636

Figure Lengend Snippet: Mounting incubator assembly (A) Representative diagram of complete mounting set up in incubator. (B) Deconstructed parts for mounting incubator. The top shelf is excluded.

Article Snippet: Ø1′′ Lens Mount with Internal and External SM1 Threads, 8–32 Tap , Thorlabs , LMR1S.

Techniques:

Journal: STAR Protocols

Article Title: Protocol for controlling visual experience during zebrafish development and modulation of motor behavior

doi: 10.1016/j.xpro.2023.102636

Figure Lengend Snippet: Rear support construction (A) The rear support baseplate (TR2) assembly is fastened to the breadboard using ¼”-20 low profile channel screws (SH25LP63 5/8″ length). Inlay (Cyan) depicts the location where screws should be inserted. An 8–32 set screw is inserted into the middle threads of the TR2 baseplate to fasten the rear support. (B) The rear support is constructed using two 1.5″ diameter, 14″ length, stainless steel posts (PTB140) and one 1.5″ diameter, 2″ length, stainless steel post (PTB020) using 8–32 set screws. (C) Representative of completed rear support post construction.

Article Snippet: Ø1′′ Lens Mount with Internal and External SM1 Threads, 8–32 Tap , Thorlabs , LMR1S.

Techniques: Construct

Journal: STAR Protocols

Article Title: Protocol for controlling visual experience during zebrafish development and modulation of motor behavior

doi: 10.1016/j.xpro.2023.102636

Figure Lengend Snippet: Camera mount assembly (A) Camera mount assembly completed using two 6″ optical posts (TR6) inserted into a parallel clamp (RA360) and attached to the rear support with a 1.5″ diameter post mounting clamp with quick release handle (C1510). (B) Location where camera should be installed on the exposed end of the TR6 optical post for the camera mount. Inlay (blue) depict lens assembly. To the camera (UI-1540LE) attach a 1″ lens mount to hold the 12 mm C-mount camera lens. The 780 nm longpass filter (FGL780) is attached to the lens using an SM1 thread adapter (SM1A25).

Article Snippet: Ø1′′ Lens Mount with Internal and External SM1 Threads, 8–32 Tap , Thorlabs , LMR1S.

Techniques:

Journal: STAR Protocols

Article Title: Protocol for controlling visual experience during zebrafish development and modulation of motor behavior

doi: 10.1016/j.xpro.2023.102636

Figure Lengend Snippet: Light mount assembly (A) Representative light mount assembly installation. Colored boxed correspond with panels (B–E). (B) (Cyan) The 1.5″ optical post holder (PH1) is attached to the breadboard using an 8–32 set screw. This will hold the 12″ optical post (TR12). (C) (Green) The rotating clamp (TRB1) is attached to the tapered end of the 12″ optical post (TR12) depicted in (B) An An 8″ optical post (TR8) is attached to the other end of the rotating clamp. (D) (Magenta) The 8″ optical post depicted in (C) holds the rotating clamp (SWC/M) with a 6″ optical post attached to the open end. The complete assembly is oriented perpendicular to the 8″ optical post. (E) (Red) The LED assembly includes the LED (MCWHL5) connected to a 1″ diameter lens mount (LMR1S). To the lens mount, attach a 2″ tube lens with included retaining ring (SM1L20) and the 1″ HDPE cutout to the end to create diffuse illumination.

Article Snippet: Ø1′′ Lens Mount with Internal and External SM1 Threads, 8–32 Tap , Thorlabs , LMR1S.

Techniques:

Journal: STAR Protocols

Article Title: Protocol for controlling visual experience during zebrafish development and modulation of motor behavior

doi: 10.1016/j.xpro.2023.102636

Figure Lengend Snippet: T-Cube and DaqModule setup (A) Depiction of T-Cube LED controller (Thorlabs: LEDD1B). Plug in power source (Thorlabs: KPS201) to the indicated input. The LED gets plugged into “LED in”. To the center plug attach the BNC connection with a positive (+) red wire and negative (-) black wire. (B) Representative of DaqModule (National Instruments: USB-6001) pinouts. The positive wire plugs into the pin 1 port (P0.1) and the negative to the digital ground port (D GND). Refer to the pinout diagram included with the DaqModule to ensure correct placement.

Article Snippet: Ø1′′ Lens Mount with Internal and External SM1 Threads, 8–32 Tap , Thorlabs , LMR1S.

Techniques:

Journal: STAR Protocols

Article Title: Protocol for controlling visual experience during zebrafish development and modulation of motor behavior

doi: 10.1016/j.xpro.2023.102636

Figure Lengend Snippet: Embedding larvae in LMP agarose (A) Representative image of dechorionating process by using microdissection forceps to pull apart chorion so larvae can swim away freely. (B) Schematic of multiple larvae mounted in a single direction (Right side down) after anaesthetizing in 1% tricane (Millipore: E10521). (C) Embedded larve are moved to the prepared mounting incubator. (Refer to “Before you begin” steps 8–13). (D) Representative of removing larvae from agar using microdissection forceps. Inlay (red) shows recommended agar cut location (Magenta dotted line) to free larvae from the agar. (E) Location of agar removal (magenta) for embedding from 4-6 dpf to allow for adequate oxygen and ion exchange during mounting. (F) Lethality of mounting for unmounted control (N = 175) and mounted (N = 198) larvae. Controls were anesthetized for the same duration as mounted individuals, but not embedded in LMP agarose following. Lethality counts were collected at 7 dpf. (G) Same experiment as in (F) examining swim bladder inflation rates. Overinflated represents individuals who float at the surface of the water, underinflated represents individuals who cannot maintain buoyancy, and normal inflation consists of individuals who can freely swim throughout the water column.

Article Snippet: Ø1′′ Lens Mount with Internal and External SM1 Threads, 8–32 Tap , Thorlabs , LMR1S.

Techniques: Laser Capture Microdissection, Control

Journal: STAR Protocols

Article Title: Protocol for controlling visual experience during zebrafish development and modulation of motor behavior

doi: 10.1016/j.xpro.2023.102636

Figure Lengend Snippet:

Article Snippet: Ø1′′ Lens Mount with Internal and External SM1 Threads, 8–32 Tap , Thorlabs , LMR1S.

Techniques: Recombinant, Software, Stripping Membranes, Sterility